Anticorps Polyclonal de lapin anti-CD5L

CD5L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17224-1-PBS

Synonymes

hAIM, CT-2, CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, API6

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Galerie de données de validation

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  WB analysis of human plasma using 17224-1-AP (same clone as 17224-1-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 17224-1-AP (CD5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17224-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 17224-1-AP (same clone as 17224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17224-1-AP (CD5L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17224-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17224-1-AP (same clone as 17224-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD5L antibody (17224-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17224-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17224-1-PBS cible CD5L dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD5L Protéine recombinante Ag11062
• Nom complet: CD5 molecule-like
• Masse moléculaire calculée: 347 aa, 38 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC033586
• Symbole du gène: CD5L
• Identification du gène (NCBI): 922
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins (PMID: 9045627; 9892623). CD5L is a soluble protein mainly secreted mostly by macrophages in lymphoid and inflamed tissues (PMID: 15009805). It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. CD5L is involved in different processes such as metabolism, infection and autophagy (PMID: 33920819).

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