Anticorps Recombinant de lapin anti-CD6

CD6 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241471H6

N° de cat : 84166-5-PBS

Synonymes

241471H6, T-cell differentiation antigen CD6

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Galerie de données de validation

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  WB analysis of mouse spleen using 84166-5-RR (same clone as 84166-5-PBS)

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84166-5-RR (CD6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84166-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84166-5-RR (same clone as 84166-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84166-5-RR (CD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84166-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84166-5-RR (same clone as 84166-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84166-5-RR (CD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84166-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 84166-5-RR (same clone as 84166-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD6 antibody (84166-5-RR, Clone: 241471H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84166-5-PBS in a different storage buffer formulation.

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Informations sur le produit

84166-5-PBS cible CD6 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD6 Protéine recombinante Eg1401
• Nom complet: CD6 antigen
• Masse moléculaire calculée: 72kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_009852.3
• Symbole du gène: Cd6
• Identification du gène (NCBI): 12511
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD6 is a type I transmembrane glycoprotein of 105-130 kDa that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harbouring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.

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