Anticorps Recombinant de lapin anti-CD62L

CD62L Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250646B2

N° de cat : 85111-6-RR

Synonymes

L-selectin, CD62 antigen-like family member L, gp90 MEL, gp90-MEL, hLHRc

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Galerie de données de validation

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  WB analysis of Jurkat using 85111-6-RR

  Jurkat cells were subjected to SDS PAGE followed by western blot with 85111-6-RR (SELL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MJ using 85111-6-RR

  MJ cells were subjected to SDS PAGE followed by western blot with 85111-6-RR (SELL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, MJ cells

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85111-6-RR cible CD62L dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD62L Protéine recombinante Eg3006
• Nom complet: selectin L
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: NM_000655.5
• Symbole du gène: CD62L
• Identification du gène (NCBI): 6402
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

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