Anticorps Monoclonal anti-CD62L

CD62L Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

DREG56

N° de cat : CPY5-65167

Synonymes

L-selectin, L selectin, hLHRc, gp90-MEL, gp90 MEL

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Galerie de données de validation

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  FC experiment of human PBMCs using CPY5-65167

  1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD62L (DREG56) (CPY5-65167, Clone:DREG56) or PerCP-Cyanine5.5 Mouse IgG1 Isotype Control (MOPC-21) (CPY5-65124, Clone: MOPC-21). Cells were co-stained with FITC Plus Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CPY5-65167

  1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD62L (DREG56) (CPY5-65167, Clone: DREG56) (red) or PerCP-Cyanine5.5 Mouse IgG1 Isotype Control (MOPC-21) (CPY5-65124, Clone: MOPC-21) (blue). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CPY5-65167 cible CD62L dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Leucocytes de sang périphérique humain activés par PMA
• Nom complet: selectin L
• Masse moléculaire calculée: 42 kDa
• Numéro d’acquisition GenBank: BC020758
• Symbole du gène: CD62L
• Identification du gène (NCBI): 6402
• Conjugaison: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 482 nm / 690nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

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