Anticorps Polyclonal de lapin anti-CD63
CD63 Polyclonal Antibody for IF, IHC, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (5)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugaison
Non conjugué
299
N° de cat : 25682-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | human urine exosomes tissue, exosomes de sérum humain |
| Résultats positifs en IHC | tissu de mélanome malin humain, tissu d'amygdalite humain, tissu de lymphome humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | tissu de mélanome malin humain, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochimie (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below |
| WB | See 278 publications below |
| IHC | See 6 publications below |
| IF | See 13 publications below |
| IP | See 1 publications below |
| FC | See 2 publications below |
Informations sur le produit
25682-1-AP cible CD63 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | canin, Humain, Lapin, poisson-zèbre, porc, singe |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | CD63 Protéine recombinante Ag19690 |
| Nom complet | CD63 molecule |
| Masse moléculaire calculée | 26 kDa |
| Poids moléculaire observé | 28-35 kDa |
| Numéro d’acquisition GenBank | BC002349 |
| Symbole du gène | CD63 |
| Identification du gène (NCBI) | 967 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for CD63 antibody 25682-1-AP | Download protocol |
| IHC protocol for CD63 antibody 25682-1-AP | Download protocol |
| IF protocol for CD63 antibody 25682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Adv Sci (Weinh) Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury | ||
J Extracell Vesicles Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. | ||
Small Methods Stimuli-Mediated Specific Isolation of Exosomes from Blood Plasma for High-Throughput Profiling of Cancer Biomarkers. | ||
J Exp Clin Cancer Res Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Federica (Verified Customer) (11-30-2023) | I used a Leica Bond Max on a healthy Tonsil tissue. Antigen Retrival ER2 95C for 30min
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FH Nydia (Verified Customer) (06-11-2022) | The HeLa cells as well as Lamp1-expressing stem cells used in the immunostaining were transfected or alternatively transfected by splitting the cells later on the glass coverslips. In order to increase cell spreading and adhesion, the coverslips used were acid washed and treated with fibronectin. Afterwards, the cells were fixed with 4% PFA, permeabilized with 0.2% Triton X-100 in phosphate- buffered saline (PBS) for 10 minutes and for 1 hour the cells were blocked with 5% BSA in PBS. Then overnight, at 4 degrees Celsius, the primary antibodies were added followed by a wash with PBS 3 times.
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FH Guorong (Verified Customer) (03-22-2022) | Smears between 30 and 60 kDa was detected
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FH David (Verified Customer) (03-30-2020) | Used for both SH-SY5Y lysate and exosomes/ extracellular vesicles. Very good signal in lysates (could be used at much higher dilution). Also good signal in exosomes, which are notoriously difficult to assess by immunoblot. Very impressive results compared to other markers.
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