Anticorps Polyclonal de lapin anti-CD63

• Phare
• Validé par KD/KO

CD63 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25682-1-PBS

Synonymes

CD 63, CD63 antigen, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1

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Galerie de données de validation

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  WB analysis of human urine exosomes using 25682-1-AP (same clone as 25682-1-PBS)

  human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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  WB analysis using 25682-1-AP (same clone as 25682-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human serum exosomes using 25682-1-AP (same clone as 25682-1-PBS)

  Human serum exosomes were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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  WB analysis using 25682-1-AP (same clone as 25682-1-PBS)

  Human plasma and human plasma-derived exosomes (human plasma-exo) were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 25682-1-AP (same clone as 25682-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD63 antibody (25682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25682-1-PBS cible CD63 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD63 Protéine recombinante Ag19690
• Nom complet: CD63 molecule
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 30-60 kDa
• Numéro d’acquisition GenBank: BC002349
• Symbole du gène: CD63
• Identification du gène (NCBI): 967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

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