Anticorps Polyclonal de lapin anti-CD63

CD63 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 32151-1-AP

Synonymes

LAMP-3 , CD63 antigen

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Galerie de données de validation

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  WB analysis using 32151-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32151-1-AP (CD63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 32151-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CD63 antibody (32151-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HUVEC, cellules K-562
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

32151-1-AP cible CD63 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD63 antigen
• Masse moléculaire calculée: 26kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: NM_001042580.1
• Symbole du gène: Cd63
• Identification du gène (NCBI): 12512
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD63, also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491), is a member of the four-times transmembrane protein superfamily (TM4SF), which constitutes a major component of lysosomal membranes. CD63 is used as a marker for exocytosis, is involved in cellular protein sorting of late endosomes and multivesicular bodies, facilitates exosome formation, and plays a role in activating ITGB1 and integrin signaling. Furthermore, CD63 is involved in intercellular adhesion through interactions with other cell surface molecules.

Publications

Species	Application	Title
mouse	WB	Theranostics; Integrating oxygen-boosted sonodynamic therapy and ferroptosis via engineered exosomes for effective cancer treatment; Authors - Mingbo Wu
mouse	WB	Adv Healthc Mater; Tumor-Resident Intracellular Bacteria Scavenger Activated In Situ Vaccines for Potent Cancer Photoimmunotherapy; Authors - Bai Lv
mouse	WB	Mol Med Rep; Exosomes derived from baicalin‑pretreated mesenchymal stem cells mitigate atherosclerosis by regulating the SIRT1/NF‑κB signaling pathway; Authors - Xiaochun Yang

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