Anticorps Monoclonal anti-CD63

CD63 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(91)

CloneNo.

3D4D1

Avis (2)

N° de cat : 67605-1-Ig

Synonymes

3D4D1, CD 63, CD63 antigen, Limp1, Lysosomal-associated membrane protein 3

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Galerie de données de validation

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  WB analysis using 67605-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 67605-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human malignant melanoma using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 40x lens).

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  IF Staining of human tonsillitis using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human lymphoma using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human lymphoma using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human malignant melanoma using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human malignant melanoma using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HUVEC, cellules K-562, cellules LNCaP, cellules MCF-7, cellules THP-1, cellules U2OS
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu de mélanome malin humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain, tissu de lymphome humain, tissu de mélanome malin humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:10000
• Immunohistochimie (IHC): IHC : 1:350-1:1400
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 85 publications below
• IF: See 5 publications below

Informations sur le produit

67605-1-Ig cible CD63 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Chèvre, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD63 Protéine recombinante Ag19690
• Nom complet: CD63 molecule
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002349
• Symbole du gène: CD63
• Identification du gène (NCBI): 967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

Publications

Species	Application	Title
human	WB	Mol Cancer; Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity; Authors - Can Can
human	WB,IF	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
human		Nat Commun; Lactate dehydrogenase A regulates tumor-macrophage symbiosis to promote glioblastoma progression; Authors - Fatima Khan
	WB	Int J Oral Sci; Administration of Porphyromonas gingivalis in pregnant mice enhances glycolysis and histone lactylation/ADAM17 leading to cleft palate in offspring; Authors - Xige Zhao
rat	IF	Bone Res; Interorgan communication in neurogenic heterotopic ossification: the role of brain-derived extracellular vesicles; Authors - Weicheng Lu
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marion (Verified Customer) (11-24-2024)

CD63 stains intracellular endosomal vesiclesd in neuronal extensions. We are satisfied with the results.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/600
• Cell Tissue Type: Mouse Brain

CD63 Antibody Immunofluorescence validation (1/600 dilution) in Mouse Brain (Cat no:67605-1-Ig)

FH

Guorong (Verified Customer) (03-22-2022)

Two bands at 30 and 60 kDa was detected

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: ARPE-19

CD63 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:67605-1-Ig)

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