Anticorps Monoclonal anti-CD63

CD63 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3D4D1

N° de cat : CL594-67605

Synonymes

CD63, CD63 antigen, CD63 molecule, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30



Applications testées

Résultats positifs en IFtissu d'amygdalite humain,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67605 cible CD63 dans les applications de IF et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène CD63 Protéine recombinante Ag19690
Nom complet CD63 molecule
Masse moléculaire calculée 26 kDa
Poids moléculaire observé 35 kDa
Numéro d’acquisition GenBankBC002349
Symbole du gène CD63
Identification du gène (NCBI) 967
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

Protocole

Product Specific Protocols
IF protocol for CL594 CD63 antibody CL594-67605Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Amr (Verified Customer) (10-07-2021)

Anti CD63 CL488 and CL594 were validated in Blood plasma exosomes and in bone marrow. The antibodies seem to recogize their specific epitopes very well both extra and intracellularly. Strongly recommended.!

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 0.5:10 CL495 and 1:10 CL488
  • Cell Tissue Type: Blood plasma/ Bone marrow
CL594 CD63 Antibody Flow Cytometry validation (0.5:10 CL495 and 1:10 CL488 dilution) in Blood plasma/ Bone marrow (Cat no:CL594-67605)