Anticorps Polyclonal de lapin anti-CD64

CD64 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27563-1-PBS

Synonymes

CD64A, Fc gamma RI, Fc gamma RIA, FCG1, Fc-gamma RI

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Galerie de données de validation

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  WB analysis using 27563-1-AP (same clone as 27563-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 27563-1-AP (same clone as 27563-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 27563-1-AP (same clone as 27563-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 27563-1-AP (same clone as 27563-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD64 antibody (27563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27563-1-PBS cible CD64 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD64 Protéine recombinante Ag26727
• Nom complet: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Masse moléculaire calculée: 374 aa, 43 kDa
• Poids moléculaire observé: 65-70 kDa, 43-45 kDa
• Numéro d’acquisition GenBank: BC032634
• Symbole du gène: FCGR1A
• Identification du gène (NCBI): 2209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.

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