Anticorps Polyclonal de lapin anti-CD68

CD68 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(102)

Avis (3)

N° de cat : 25747-1-AP

Synonymes

CD68 molecule, GP110, Macrosialin, SCARD1

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Galerie de données de validation

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  WB analysis using 25747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25747-1-AP (CD68 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 25747-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25747-1-AP (CD68 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 25747-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25747-1-AP (CD68 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25747-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25747-1-AP (CD68 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25747-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25747-1-AP (CD68 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25747-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25747-1-AP (CD68 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 25747-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CD68 antibody (25747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon using 25747-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CD68 antibody (25747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 25747-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD68 antibody (25747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 25747-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD68 antibody (25747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human appendicitis using 25747-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD68 antibody (25747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human appendicitis using 25747-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD68 antibody (25747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules U-937
• Résultats positifs en IHC: tissu hépatique humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon humain, tissu d'amygdalite humain, tissu d'appendicite humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 46 publications below
• IF: See 45 publications below

Informations sur le produit

25747-1-AP cible CD68 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, poisson-zèbre, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD68 Protéine recombinante Ag22815
• Nom complet: CD68 molecule
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC015557
• Symbole du gène: CD68
• Identification du gène (NCBI): 968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.

Publications

Species	Application	Title
		Cell Metab; Dual impacts of serine/glycine-free diet in enhancing antitumor immunity and promoting evasion via PD-L1 lactylation; Authors - Huan Tong
		Cell Metab; Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species.; Authors - Xiao-Jing Zhang
human	IF	Theranostics; Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages; Authors - Xueqin Li
	IHC	Nat Commun; The ubiquitin ligase ZNRF1 promotes caveolin-1 ubiquitination and degradation to modulate inflammation.; Authors - Chih-Yuan Lee
	IF	EBioMedicine; Social stress is lethal in the mdx model of Duchenne muscular dystrophy.; Authors - Maria Razzoli
	IF	Aging Cell; Inhibition of DNA methyltransferase aberrations reinstates antioxidant aging suppressors and ameliorates renal aging.; Authors - Qi Gao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zach (Verified Customer) (09-12-2025)

The antibody works perfectly with liver samples of MASH mice, and pancreas samples of chronic pancreatitis mice.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreas, liver

FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue with a Tris-EDTA antigen retrieval. Also works by IHC.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Prostate

CD68 Antibody Immunohistochemistry,Immunofluorescence validation (1:200 dilution) in Prostate (Cat no:25747-1-AP)

FH

Fabio Henrique (Verified Customer) (01-17-2019)

Figure 1. IF of CD68 (red) in human spleen. Formalin-fixed paraffin-embedded human spleen tissue was used as positive control to probe for CD68. Heat-induced antigen retrieval was performed in sodium citrate buffer pH 6.0 + Tween20 at 0.5%. Tissue incubated at 96*C for 20 min inside the buffer. Permeabilization was done with washes of TBSTritonX 0.25% for 3x 5min and blocking was done in TBS 10% Goat serum, 1% BSA, for 1 hr at RT. Anti-CD68 was used at 1:200 in blocking buffer, overnight incubation at 4*C. Secondary antibody AlexaFluor 488 was used at 1:500 for 1 hr at RT. Imaged using a Nikon A1 confocal microscope. Figure 2: CD68 (red) and CD206 (green) in human primary macrophages polarized to M2-phenotype, encapsulated in 3D hydrogel (hyaluronic acid and collagen type 1). Staining was performed as described above, except primary (CD68) was used at 1:100 and secondary antibodies were used at 1:500.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100 (hydrogels); 1:200 (2D samples)
• Cell Tissue Type: Spleen and 3D culture of human primary macrophages

CD68 Antibody Immunofluorescence, validation (1:100 (hydrogels); 1:200 (2D samples) dilution) in Spleen and 3D culture of human primary macrophages (Cat no:25747-1-AP)

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