Anticorps Polyclonal de lapin anti-CD68

CD68 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30929-1-PBS

Synonymes

CD68 antigen, gp110, Scard1

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Galerie de données de validation

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  WB analysis using 30929-1-AP (same clone as 30929-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30929-1-AP (CD68 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30929-1-PBS cible CD68 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD68 Protéine recombinante Eg0787
• Nom complet: CD68 antigen
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: NM_001291058.1
• Symbole du gène: Cd68
• Identification du gène (NCBI): 12514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

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