Anticorps Recombinant de lapin anti-CD68

CD68 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

KP1

N° de cat : 65593-1-PBS

Synonymes

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

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Galerie de données de validation

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  FC experiment of human PBMCs using 65593-1-MR (same clone as 65593-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone: KP1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 65593-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65593-1-MR (same clone as 65593-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 65593-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65593-1-MR (same clone as 65593-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (left) or 0.25 ug Anti-Human CD68 (65202-1-Ig, Clone: KP1) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 65593-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65593-1-PBS cible CD68 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD68 molecule
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: BC015557
• Symbole du gène: CD68
• Identification du gène (NCBI): 968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

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