Anticorps Recombinant de lapin anti-CD68

CD68 Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230504E9

N° de cat : 83014-2-PBS

Synonymes

CD68 antigen, gp110, Scard1

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Galerie de données de validation

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  Sandwich ELISA sample test of MP00015-4

  NIH/3T3 mouse embryo fibroblasts were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse CD68 and measured 3.5 pg/mL. J774A.1 mouse reticulum cell sarcoma macrophage cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse CD68 and measured 1,337.7 pg/mL.

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  Sandwich ELISA sample test of MP00015-4

  The mouse CD68 concentration was determined to be 378,104.1 pg/mL in RAW264.7 cell extract based on a 4.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP00015-4

  Sandwich ELISA standard curve of MP00015-4, Mouse CD68 Recombinant Matched Antibody Pair - PBS only. 83014-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0787. 83014-6-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

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  Affinity and Kinetic Characterization of 83014-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83014-2-PBS against Mouse CD68 was performed. The affinity constant is 1.30 nM.

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Informations sur le produit

83014-2-PBS cible CD68 dans les applications de Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD68 Protéine recombinante Eg0787
• Nom complet: CD68 antigen
• Masse moléculaire calculée: 35 kDa
• Numéro d’acquisition GenBank: NM_001291058.1
• Symbole du gène: Cd68
• Identification du gène (NCBI): 12514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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