Anticorps Recombinant de lapin anti-CD68

CD68 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

230504G10

N° de cat : 83014-5-RR

Synonymes

Scard1, gp110, CD68 antigen, 230504G10

---

Galerie de données de validation

• 

  WB analysis using 83014-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83014-5-RR (CD68 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 83014-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 83014-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 83014-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 83014-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 83014-5-RR

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 83014-5-RR

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 83014-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 83014-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat small intestine using 83014-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83014-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83014-5-RR against Mouse CD68 were performed. The affinity constant is 8.77 nM.

---

Applications testées

• Résultats positifs en WB: cellules RAW 264.7, J774A.1 cells, NR8383 cells
• Résultats positifs en IHC: tissu splénique de souris, tissu d'intestin grêle de rat, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris, tissu d'intestin grêle de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

83014-5-RR cible CD68 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD68 Protéine recombinante Eg0787
• Nom complet: CD68 antigen
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: NM_001291058.1
• Symbole du gène: Cd68
• Identification du gène (NCBI): 12514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
mouse	IHC	Front Pharmacol; Clusterin ameliorates diabetic atherosclerosis by suppressing macrophage pyroptosis and activation; Authors - Lingling Xuan

{{ptg:RelatedPrimaryAntibodies}}