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Anticorps Monoclonal anti-CD68

CD68 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

APC Fluorescent Dye

CloneNo.

Y1/82A

N° de cat : APC-65187

Synonymes

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

Galerie de données de validation

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  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD68 (Y1/82A) (APC-65187)

    FC experiment of human PBMCs using APC-65187

    1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD68 (Y1/82A) (APC-65187)

    FC experiment of human PBMCs using APC-65187

    1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.

Applications testées

Résultats positifs en cytométrieCMSP humaines

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65187 cible CD68 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2b, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Contenu lysosomal des macrophages pulmonaires
Nom complet CD68 molecule
Masse moléculaire calculée 37 kDa
Numéro d’acquisition GenBankBC015557
Symbole du gène CD68
Identification du gène (NCBI) 968
Conjugaison APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Forme Liquide
Méthode de purification L'anticorps purifié est conjugué à l'allophycocyanine (APC) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
Tampon de stockage PBS avec azoture de sodium à 0,1 %.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Protocole

Product Specific Protocols
FC protocol for APC CD68 antibody APC-65187Download protocol
Standard Protocols
Click here to view our Standard Protocols