Anticorps Polyclonal de lapin anti-CD69

CD69 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 10803-1-AP

Synonymes

CLEC2C, CD69 molecule, BL-AC/P26, BL AC/P26, AIM

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Galerie de données de validation

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  WB analysis using 10803-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 10803-1-AP (CD69 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 10803-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10803-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10803-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10803-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 10803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: plaquettes de sang périphérique humain,
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• FC: See 1 publications below

Informations sur le produit

10803-1-AP cible CD69 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD69 Protéine recombinante Ag1094
• Nom complet: CD69 molecule
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 28 kDa, 32 kDa
• Numéro d’acquisition GenBank: BC007037
• Symbole du gène: CD69
• Identification du gène (NCBI): 969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD69, also known as activation inducer molecule (AIM) or early activation antigen (EA1), is a cell surface glycoprotein expressed shortly after activation on T lymphocytes and other cells of haematopoietic origin, including natural killer cells. It is a disulfide-linked homodimer composed of two chains belonging to the type II integral protein with a C-type lectin domain. CD69 is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, natural killer cells, and platelets.

Publications

Species	Application	Title
human	IHC	Cell Stem Cell; Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy; Authors - Meghan Logun
	IHC	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
human	IF	iScience; Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis; Authors - Mick J M van Eijs
human	IF	Expert Rev Clin Immunol; Juxtaposition of IL-1β and IFN-γ expression and apoptosis of keratinocytes in adult-onset Still's disease.; Authors - Shijia Rao
	IF	Cell Rep; Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.; Authors - Huiwen Zheng
human	IHC	Oncoimmunology; Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.; Authors - Chen Zhu

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