Anticorps Recombinant de lapin anti-CD69

CD69 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241552A2

N° de cat : 84258-7-RR

Synonymes

241552A2, Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26

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Galerie de données de validation

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  WB analysis using 84258-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84258-7-RR (CD69 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84258-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84258-7-RR against Human CD69 were performed. The affinity constant is 0.776 nM.

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84258-7-RR cible CD69 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD69 Protéine recombinante Eg1791
• Nom complet: CD69 molecule
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_001781.2
• Symbole du gène: CD69
• Identification du gène (NCBI): 969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

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