Anticorps Recombinant de lapin anti-CD69

CD69 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241552G10

N° de cat : 98173-2-RR

Synonymes

Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule

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Galerie de données de validation

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  FC experiment of human PBMCs using 98173-2-RR

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-2-RR, Clone: 241552F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD4 (RPA-T4). Cells were not fixed.

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  FC experiment of human PBMCs using 98173-2-RR

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-2-RR, Clone: 241552F1) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-2-RR, Clone:241552F1)(black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of Cnyo PBMCs using 98173-2-RR

  1x10^6 Cnyo PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit RecAb (98173-2-RR, Clone:241552G10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  FC experiment of Cnyo PBMCs using 98173-2-RR

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit RecAb (98173-2-RR, Clone: 241552G10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs, cynomolgus PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98173-2-RR cible CD69 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD69 Protéine recombinante Eg1791
• Nom complet: CD69 molecule
• Masse moléculaire calculée: 23 kDa
• Numéro d’acquisition GenBank: NM_001781.2
• Symbole du gène: CD69
• Identification du gène (NCBI): 969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

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