Anticorps Recombinant de lapin anti-CD69

CD69 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

241552F1

N° de cat : CL750-98173

Synonymes

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

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Galerie de données de validation

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  FC experiment of human PBMCs using CL750-98173

  1x10^6 untreated or PHA treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD4 Rabbit Recombinant Antibody (CL488-98042, Clone: 240427E12) and 5 ul CoraLite® Plus 750 Anti-Human CD69 Rabbit Recombinant Antibody (CL750-98173, Clone: 241552F1). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL750-98173

  1x10^6 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD69 Rabbit Recombinant Antibody (CL750-98173, Clone: 241552F1) (red) or CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD69 Rabbit Recombinant Antibody (CL750-98173, Clone:241552F1) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-98173 cible CD69 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD69 molecule
• Masse moléculaire calculée: 23 kDa
• Numéro d’acquisition GenBank: NM_001781.2
• Symbole du gène: CD69
• Identification du gène (NCBI): 969
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

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