Anticorps Monoclonal anti-CD7

CD7 Monoclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1G10D8

N° de cat : 60209-1-Ig

Synonymes

1G10D8, CD7 molecule, GP40, LEU-9, T cell antigen CD7

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 60209-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-1-Ig (CD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 60209-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 60209-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60209-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60209-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 60209-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-1-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 60209-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-1-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human tonsillitis using 60209-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60209-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en IHC: tissu d'appendicite humain, tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1600-1:6400
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

60209-1-Ig cible CD7 dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD7 Protéine recombinante Ag1805
• Nom complet: CD7 molecule
• Masse moléculaire calculée: 240 aa, 25 kDa
• Numéro d’acquisition GenBank: BC009293
• Symbole du gène: CD7
• Identification du gène (NCBI): 924
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).

Publications

Species	Application	Title
	IF	Cell Metab; Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain; Authors - Hai-Yan Zhou
		Small; In Vivo mRNA CAR T Cell Engineering via Targeted Ionizable Lipid Nanoparticles with Extrahepatic Tropism; Authors - Margaret M Billingsley

{{ptg:RelatedPrimaryAntibodies}}