Anticorps Monoclonal anti-CD7

CD7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A4E6

N° de cat : 60209-2-PBS

Synonymes

2A4E6, CD7 molecule, GP40, LEU-9, T cell antigen CD7

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Galerie de données de validation

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  WB analysis of Jurkat using 60209-2-Ig (same clone as 60209-2-PBS)

  Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  WB analysis using 60209-2-Ig (same clone as 60209-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse thymus using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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Informations sur le produit

60209-2-PBS cible CD7 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD7 Protéine recombinante Ag1805
• Nom complet: CD7 molecule
• Masse moléculaire calculée: 240 aa, 25 kDa
• Poids moléculaire observé: 34-36 kDa
• Numéro d’acquisition GenBank: BC009293
• Symbole du gène: CD7
• Identification du gène (NCBI): 924
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).

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