Anticorps Monoclonal anti-CD7

CD7 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Cardinal Red™ Fluorescent Dye

CloneNo.

4H9

N° de cat : CR-65203

Synonymes

4H9, CD7 molecule, GP40, LEU-9, T cell antigen CD7

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Galerie de données de validation

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  FC experiment of human PBMCs using CR-65203

  1X10^6 human PBMCs were surface co-stained with APC Anti-Human CD4 and 5 ul Cardinal Red™ Anti-Human CD7 (CR-65203, Clone:4H9) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CR-65203

  1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD7 (CR-65203, Clone:4H9) (red) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CR-65203 cible CD7 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules de leucémie lymphoblastique aiguë T
• Nom complet: CD7 molecule
• Masse moléculaire calculée: 240 aa, 25 kDa
• Numéro d’acquisition GenBank: BC009293
• Symbole du gène: CD7
• Identification du gène (NCBI): 924
• Conjugaison: Cardinal Red™ Fluorescent Dye
• Excitation/Emission maxima wavelengths: 592 nm / 611 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).

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