Anticorps Recombinant de lapin anti-CD70

CD70 Recombinant Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241324B12

N° de cat : 84455-2-RR

Synonymes

241324B12, CD27 L, CD27 ligand, CD27L, CD27-L

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Galerie de données de validation

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  IF Staining of THP-1 using 84455-2-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD70 antibody (84455-2-RR, Clone: 241324B12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84455-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84455-2-RR against Human CD70 were performed. The affinity constant is 67.0 pM.

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Applications testées

• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84455-2-RR cible CD70 dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD70 molecule
• Masse moléculaire calculée: 21 kDa
• Numéro d’acquisition GenBank: BC000725
• Symbole du gène: CD70
• Identification du gène (NCBI): 970
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD70, also known as tumor necrosis factor ligand superfamily member 7 (TNFSF7) or CD27 ligand (CD27L), is a transmembrane protein that plays a significant role in the immune system. It is the unique ligand for CD27, a member of the tumor necrosis factor receptor (TNFR) family, and is transiently upregulated upon stimulation of immune cells. CD70 is expressed on lymphocytes and dendritic cells, and its expression can be regulated by activation signals received via toll-like receptors (TLRs), CD40, and the antigen receptor MHC II. It provides complex and not completely understood signaling for B cell development. CD70 is also expressed on various solid tumors and hematolymphoid malignancies, where it may contribute to a poor prognosis.

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