Anticorps Monoclonal anti-CD71

CD71 Monoclonal Antibody for WB, IHC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC

Conjugaison

Non conjugué

Publications(5)

CloneNo.

H68.4

N° de cat : 65236-1-Ig

Synonymes

H68.4, p90, sTfR, T9, TFR

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Galerie de données de validation

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  WB analysis using 65236-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 65236-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 65236-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 65236-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa, cellules K-562, cellules RAW 264.7
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

65236-1-Ig cible CD71 dans les applications de WB, IHC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: transferrin receptor (p90, CD71)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC001188
• Symbole du gène: CD71
• Identification du gène (NCBI): 7037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publications

Species	Application	Title
mouse	IHC	Mol Med; TIMP1 regulates ferroptosis in osteoblasts by inhibiting TFRC ubiquitination: an in vitro and in vivo study; Authors - Bo Peng
mouse	WB	J Ethnopharmacol; The extract from Quzhou Aurantii Fructus attenuates cough variant asthma through inhibiting the TRPV1/Ca2+/NFAT/TSLP pathway and ferroptosis via TRPV1 mediation in ovalbumin-induced mice; Authors - Meizi Tian
rat	WB	Hum Exp Toxicol; KLF6 silencing attenuates MCAO-induced brain injury and cognitive dysfunction via targeting ferroptosis and activating the Nrf2/HO-1 pathway; Authors - Hongyu Qian
	WB	Int Immunopharmacol; Urolithin A improves myocardial ischemia-reperfusion injury by attenuating oxidative stress and ferroptosis through Nrf2 pathway; Authors - Zhe Su
human	WB	Front Neurol; Cynaroside: a potential therapeutic agent targeting arachidonate 15-lipoxygenase to mitigate cerebral ischemia/reperfusion injury; Authors - Wenpeng Cao

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