Anticorps Recombinant de lapin anti-CD71

• Phare
• Validé par KD/KO

CD71 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2P14

N° de cat : 81793-2-PBS

Synonymes

2P14, p90, sTfR, T9, TFR

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Galerie de données de validation

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  WB analysis using 81793-2-RR (same clone as 81793-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81793-2-RR (CD71 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81793-2-RR (same clone as 81793-2-PBS)

  WB result of CD71 antibody (81793-2-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD71 transfected HeLa cells. This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81793-2-RR (same clone as 81793-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81793-2-RR (CD71 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 81793-2-RR (same clone as 81793-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

• 

  IF Staining of K-562 using 81793-2-RR (same clone as 81793-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81793-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81793-2-RR against Human CD71 were performed. The affinity constant is 2.85 nM.

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Informations sur le produit

81793-2-PBS cible CD71 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD71 Protéine recombinante Ag0123
• Nom complet: transferrin receptor (p90, CD71)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC001188
• Symbole du gène: CD71
• Identification du gène (NCBI): 7037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

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