Anticorps Recombinant de lapin anti-CD71

• Phare
• Validé par KD/KO

CD71 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2P14

N° de cat : 81793-2-RR

Synonymes

2P14, p90, sTfR, T9, TFR

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Galerie de données de validation

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  WB analysis using 81793-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81793-2-RR (CD71 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 81793-2-RR

  WB result of CD71 antibody (81793-2-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD71 transfected HeLa cells.

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  WB analysis using 81793-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81793-2-RR (CD71 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 81793-2-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of K-562 using 81793-2-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 81793-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81793-2-RR against Human CD71 were performed. The affinity constant is 2.85 nM.

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules DU 145, cellules HeLa, cellules HT-1080, cellules K-562, cellules PC-3, cellules Raji
• Résultats positifs en IF/ICC: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

81793-2-RR cible CD71 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD71 Protéine recombinante Ag0123
• Nom complet: transferrin receptor (p90, CD71)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC001188
• Symbole du gène: CD71
• Identification du gène (NCBI): 7037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; NLRP3 regulates ferroptosis via the JAK2/STAT3 pathway in asthma inflammation: Insights from in vivo and in vitro studies; Authors - Zhengze Zhang
mouse	IF	J Nanobiotechnology; Microglia-targeting nanosystems that cooperatively deliver Chinese herbal ingredients alleviate behavioral and cognitive deficits in Alzheimer's disease model mice; Authors - Peng Zhou

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