Anticorps Recombinant de lapin anti-CD71

CD71 Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250738C5

N° de cat : 81793-3-PBS

Synonymes

p90, sTfR, T9, TFR, TFR1

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Galerie de données de validation

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  IHC staining of human placenta using 81793-3-RR (same clone as 81793-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81793-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 81793-3-RR (same clone as 81793-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81793-3-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 81793-3-RR (same clone as 81793-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-3-RR, Clone: 250738C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81793-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81793-3-RR

  Biolayer interferometry (BLl) kinetic assays of 81793-3-RR against Human CD71 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

81793-3-PBS cible CD71 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD71 Protéine recombinante Eg0339
• Nom complet: transferrin receptor (p90, CD71)
• Masse moléculaire calculée: 85 kDa
• Symbole du gène: CD71
• Identification du gène (NCBI): 7037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

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