Anticorps Recombinant de lapin anti-CD71

CD71 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

242122B2

N° de cat : 84766-4-RR

Synonymes

Tfrc, transferrin receptor, TR, TFR1, TFR

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Galerie de données de validation

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  WB analysis using 84766-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84766-4-RR (Transferrin R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 84766-4-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD71 antibody (84766-4-RR, Clone: 242122B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84766-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84766-4-RR against Mouse CD71 were performed. The affinity constant is 1.04 nM.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, tissu placentaire de souris
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

84766-4-RR cible CD71 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: transferrin receptor
• Masse moléculaire calculée: 86kDa
• Poids moléculaire observé: 85-100 kDa
• Numéro d’acquisition GenBank: NM_011638.4
• Symbole du gène: CD71
• Identification du gène (NCBI): 22042
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publications

Species	Application	Title
mouse	WB	Toxicology; Benzyl butyl phthalate promotes ferroptosis in Sertoli cells via disrupting ceruloplasmin-mediated iron balance; Authors - Yu Dong
mouse	WB	Biomed Pharmacother; p16INK4a downregulation alleviates temporomandibular joint osteoarthritis combined with type 2 diabetes by driving M2 polarization; Authors - Jilin Cai

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