Anticorps Monoclonal anti-CD71

CD71 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

OKT9

N° de cat : CL555-65235

Synonymes

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

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Galerie de données de validation

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  FC experiment of human PBMCs using CL555-65235

  1X10^6 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD71 (CL555-65235, Clone: OKT9) (red) or Mouse IgG1 Isotype Control (blue). Unactivated PBMCs were surface stained with CL555-65235 (black). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL555-65235

  1X10^6 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD71 (CL555-65235, Clone: OKT9) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65235 cible CD71 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Human T-cell acute lymphoblastic leukemia cells
• Nom complet: transferrin receptor (p90, CD71)
• Masse moléculaire calculée: 85 kDa
• Numéro d’acquisition GenBank: BC001188
• Symbole du gène: CD71
• Identification du gène (NCBI): 7037
• Conjugaison: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

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