Anticorps Recombinant de lapin anti-CD71

CD71 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

OKT9

N° de cat : FITC-65507

Synonymes

OKT9, p90, sTfR, T9, TFR

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65507

  1X10^6 PHA-activated (3 day) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul FITC Plus Anti-Human CD71 Rabbit Recombinant Antibody (FITC-65507, Clone:OKT9) (right) or FITC Plus-conjugated Rabbit IgG control PolyAb (FITC-30000) (left). 1X10^6 unactivated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD71 Mouse Monoclonal Antibody (center). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65507

  1X10^6 PHA-activated (3 day) human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD71 Rabbit Recombinant Antibody (FITC-65507, Clone:OKT9) (red) or FITC Plus-conjugated Rabbit IgG control PolyAb (FITC-30000) (blue). 1X10^6 unactivated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD71 Mouse Monoclonal Antibody (black dashed). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65507

  1X10^6 PHA-activated (3 day) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul FITC Plus Anti-Human CD71 Rabbit Recombinant Antibody (FITC-65507, Clone:OKT9) (left) or 5 ul FITC Plus Anti-Human CD71 Mouse Monoclonal Antibody (FITC-65235, Clone:OKT9) (right). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: PHA treated (3 day) human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65507 cible CD71 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Human T-cell acute lymphoblastic leukemia cells
• Nom complet: transferrin receptor (p90, CD71)
• Masse moléculaire calculée: 85 kDa
• Numéro d’acquisition GenBank: BC001188
• Symbole du gène: CD71
• Identification du gène (NCBI): 7037
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

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