Anticorps Recombinant de lapin anti-CD72

CD72 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242846B5

N° de cat : 85208-5-PBS

Synonymes

242846B5, B-cell differentiation antigen CD72, Ly32, Ly-32, Lyb-2

---

Galerie de données de validation

• 

  WB analysis of mouse spleen using 85208-5-RR (same clone as 85208-5-PBS)

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 85208-5-RR (Cd72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85208-5-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 85208-5-RR (same clone as 85208-5-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85208-5-RR (Cd72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85208-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 85208-5-RR (same clone as 85208-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85208-5-RR (CD72 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85208-5-PBS in a different storage buffer formulation.

---

Informations sur le produit

85208-5-PBS cible CD72 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD72 Protéine recombinante Eg2708
• Nom complet: CD72 antigen
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_007654.2
• Symbole du gène: Cd72
• Identification du gène (NCBI): 12517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

{{ptg:RelatedPrimaryAntibodies}}