Anticorps Recombinant de lapin anti-CD73

CD73 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

AD2

N° de cat : 65500-1-RR

Synonymes

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

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Galerie de données de validation

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  FC experiment of human PBMCs using 65500-1-RR

  1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) or Rabbit IgG control PolyAb. Unconjugated antibodies were labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65500-1-RR

  1X10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) or Rabbit IgG control PolyAb. Unconjugated antibodies were labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65500-1-RR

  1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) (left) or 0.4 ug Anti-Human CD73 Mouse Monoclonal Antibody (Clone:AD2) (center and right). Each unconjugated primary antibody was labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) or FlexAble CL750 Antibody Labeling Kit for Mouse IgG1 (KFA024) prior to staining. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65500-1-RR cible CD73 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Pre-B leukemia cell line
• Nom complet: 5'-nucleotidase, ecto (CD73)
• Masse moléculaire calculée: 29 kDa, 63 kDa
• Numéro d’acquisition GenBank: BC015940
• Symbole du gène: NT5E
• Identification du gène (NCBI): 4907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).