Anticorps Monoclonal anti-CD74

CD74 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D8A1

N° de cat : 66390-1-PBS

Synonymes

Ia antigen-associated invariant chain, HLA-DR antigens-associated invariant chain, HLADG, DHLAG, Class-II-associated invariant chain peptide

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Galerie de données de validation

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  WB analysis using 66390-1-Ig (same clone as 66390-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66390-1-Ig (same clone as 66390-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66390-1-Ig (same clone as 66390-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 66390-1-Ig (same clone as 66390-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD74 antibody (66390-1-Ig, Clone: 2D8A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66390-1-PBS cible CD74 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD74 Protéine recombinante Ag25503
• Nom complet: CD74 molecule, major histocompatibility complex, class II invariant chain
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC018726
• Symbole du gène: CD74
• Identification du gène (NCBI): 972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165;PMID: 36712241).

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