Anticorps Polyclonal de lapin anti-CD79b

CD79b Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 21063-1-AP

Synonymes

Ig-beta, IGB, Ig beta, B-cell-specific glycoprotein B29, B-cell antigen receptor complex-associated protein beta chain

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Galerie de données de validation

• 

  WB analysis of Raji using 21063-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21063-1-AP (CD79B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79b antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de côlon de souris, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 4 publications below

Informations sur le produit

21063-1-AP cible CD79b dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD79b Protéine recombinante Ag15086
• Nom complet: CD79b molecule, immunoglobulin-associated beta
• Masse moléculaire calculée: 229 aa, 26 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC032651
• Symbole du gène: CD79B
• Identification du gène (NCBI): 974
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).

Publications

Species	Application	Title
human	IF	Front Immunol; CD20+CD22+ADAM28+ B Cells in Tertiary Lymphoid Structures Promote Immunotherapy Response.; Authors - Zhenghao Wu
mouse	IF	Cell Discov; Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure; Authors - Jie Yin
human	IF	J Nanobiotechnology; Targeted degradation of oncogenic BCR-ABL by silencing the gene of NEDD8 E3 ligase RAPSYN; Authors - Yanzi Sun
human	IF	Int Endod J; FoxO1 mediates odontoblast differentiation of hDPSCs via B cell-derived ANGPTL1 in dental caries: A laboratory investigation; Authors - Peimeng Zhan

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