Anticorps Polyclonal de lapin anti-CD79a

CD79a Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31887-1-PBS

Synonymes

B-cell antigen receptor complex-associated protein alpha chain, Mb-1

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Galerie de données de validation

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  WB analysis using 31887-1-AP (same clone as 31887-1-PBS)

  Mouse spleen tissue was subjected to SDS PAGE, followed by western blot with 31887-1-AP (CD79a antibody) at a dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31887-1-AP (same clone as 31887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31887-1-AP (same clone as 31887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 31887-1-AP (same clone as 31887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 31887-1-AP (same clone as 31887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat spleen using 31887-1-AP (same clone as 31887-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD79a antibody (31887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31887-1-PBS cible CD79a dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD79a Protéine recombinante Eg1388
• Nom complet: CD79A antigen (immunoglobulin-associated alpha)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: NM_007655.3
• Symbole du gène: Cd79a
• Identification du gène (NCBI): 12518
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

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