Anticorps Recombinant de lapin anti-CD79a

CD79a Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241299F1

N° de cat : 84236-4-RR

Synonymes

MB-1 membrane glycoprotein, MB1, MB 1 membrane glycoprotein, MB 1, B-cell antigen receptor complex-associated protein alpha chain

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Galerie de données de validation

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  WB analysis using 84236-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84236-4-RR (CD79a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 84236-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84236-4-RR (CD79a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84236-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84236-4-RR (CD79a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84236-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84236-4-RR (CD79a antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84236-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84236-4-RR against Human CD79a were performed. The affinity constant is 20.8 nM.

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji, cellules Ramos
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84236-4-RR cible CD79a dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD79a molecule, immunoglobulin-associated alpha
• Masse moléculaire calculée: 226 aa, 25 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC113733
• Symbole du gène: CD79A
• Identification du gène (NCBI): 973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

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