Anticorps Recombinant de lapin anti-CD8

CD8 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

Biotin

CloneNo.

SK1

N° de cat : Biotin-65630

Synonymes

CD8A, CD8a molecule, Leu2, MAL, p32

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Galerie de données de validation

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  FC experiment of human PBMCs using Biotin-65630

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD8 (SK1) Mouse IgG2a RecAb (Biotin-65630, Clone: SK1), or unstained. Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-65630

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD8 (SK1) Mouse IgG2a RecAb (Biotin-65630, Clone: SK1) (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-65630 cible CD8 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Human peripheral blood T lymphocytes
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: Biotin
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

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