Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF-P

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1G2B10

N° de cat : CL594-66868

Synonymes

CD8, 1G2B10, p32, MAL, Leu2

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Galerie de données de validation

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  IF Staining of human colon cancer using CL594-66868

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10 ) at dilution of 1:200, CoraLite® Plus 488 pan-keratin antibody (CL488-26411, green), CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta).

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  IF Staining of human tonsillitis using CL594-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66868 (CD8a antibody) at dilution of 1:50.

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  IF Staining of human tonsillitis using CL594-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10 ) at dilution of 1:400. DAPI (blue).

• 

  IF Staining of human colon cancer using CL594-66868

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta) at dilution of 1:100, CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

• 

  IF Staining of human colon cancer using CL594-66868

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Cyan) at dilution of 1:100, CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

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Applications testées

• Résultats positifs en IF-P: tissu d'appendicite humain, tissu d'amygdalite humain, tissu de cancer du côlon humain

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66868 cible CD8a dans les applications de IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD8a Protéine recombinante Ag11111
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.

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