Anticorps Recombinant de lapin anti-CD8

CD8 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

CoraLux Violet 510 Fluorescent Dye

CloneNo.

SK1

N° de cat : CLV510-65630

Synonymes

CD8A, CD8a molecule, Leu2, MAL, p32

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Galerie de données de validation

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  FC experiment of human PBMCs using CLV510-65630

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3, and 5 ul CoraLux Violet 510 Anti-Human CD8 (SK1) Mouse IgG2a RecAb (CLV510-65630, Clone:SK1) or unstained. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CLV510-65630

  1x10^6 human PBMCs were surface stained with 5 ul CoraLux Violet 510 Anti-Human CD8 (SK1) Mouse IgG2a RecAb (CLV510-65630, Clone:SK1) (red) or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CLV510-65630 cible CD8 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Human peripheral blood T lymphocytes
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: CoraLux Violet 510 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 410 nm / 501 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

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