Anticorps Monoclonal anti-CD80 (B7-1)

CD80 (B7-1) Monoclonal Antibody for FC

Hôte / Isotype

Armenian Hamster / IgG

Réactivité testée

souris et plus (1)

Applications

FC

Conjugaison

PE Fluorescent Dye

Publications

3

CloneNo.

16-10A1

N° de cat : PE-65076

Synonymes

B7 1, B7.1, Cd28l, Cd80, CD80 (B7-1), CD80 antigen, Ly 53, Ly53, MIC17, TS/A 1

---

Galerie de données de validation

• 

  FC experiment of Balb/c mouse peritoneal macrophages using PE-65076

  1X10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug PE Anti-Mouse CD80 (B7-1) (PE-65076, Clone:16-10A1) (red), or 0.5 ug Isotype Control. Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: macrophages péritonéaux de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 3 publications below

Informations sur le produit

PE-65076 cible CD80 (B7-1) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Armenian Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de cellules CHO transfectées avec B7 (CD80) de souris
• Nom complet: CD80 antigen
• Numéro d’acquisition GenBank: BC131959
• Symbole du gène: Cd80
• Identification du gène (NCBI): 12519
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.1% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse	FC	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	FC	Asian J Pharm Sci; Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors; Authors - Xudong Fan
human	FC	Int Immunopharmacol; Hsa_circRNA_103124 upregulation in Crohn's disease promoted macrophage M1 polarization to maintain an inflammatory microenvironment via activation of the AKT2 and TLR4/NF-κB pathways; Authors - Juan Yin