Anticorps Recombinant de lapin anti-CD80

CD80 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250725H4

N° de cat : 86532-1-RR

Synonymes

B7 1, B7.1, B7-1, Cd80 molecule

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Galerie de données de validation

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  WB analysis of NR8383 using 86532-1-RR

  NR8383 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (CD80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86532-1-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (B7-1 / CD80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86532-1-RR

  C2C12 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (B7-1 / CD80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of C2C12 using 86532-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CD80 antibody (86532-1-RR, Clone: 250725H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: NR8383 cells, cellules C2C12, cellules RAW 264.7
• Résultats positifs en IF/ICC: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86532-1-RR cible CD80 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD80 Protéine recombinante Eg3841
• Nom complet: Cd80 molecule
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 60-70 kDa
• Symbole du gène: CD80
• Identification du gène (NCBI): 25408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

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