Anticorps Recombinant de lapin anti-CD80

CD80 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

PE-Cyanine7 Fluorescent Dye

CloneNo.

241371E12

N° de cat : PY7-98158

Synonymes

241371E12, B7 1, B7.1, Cd28l, CD80 antigen

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Galerie de données de validation

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  FC experiment of mouse peritoneal macrophages using PY7-98158

  1x10^6 mouse peritoneal macrophages were surface stained with 0.2 ug PE-Cyanine7 Anti-Mouse CD80 Rabbit RecAb (PY7-98158, Clone: 241371E12) or unstained. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using PY7-98158

  1x10^6 mouse peritoneal macrophages were surface stained with 0.2 ug PE-Cyanine7 Anti-Mouse CD80 Rabbit RecAb (PY7-98158, Clone: 241371E12)(red), or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: macrophages péritonéaux de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PY7-98158 cible CD80 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD80 Protéine recombinante Eg0840
• Nom complet: CD80 antigen
• Masse moléculaire calculée: 35 kDa
• Numéro d’acquisition GenBank: NM_009855.2
• Symbole du gène: CD80
• Identification du gène (NCBI): 12519
• Conjugaison: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 450-500 nm / 778 nm
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

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