Anticorps Monoclonal anti-CD80

CD80 Monoclonal Antibody for FC

Hôte / Isotype

Armenian Hamster / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

Publications(2)

CloneNo.

16-10A1

N° de cat : 65076-1-Ig

Synonymes

Ly53, Ly 53, CD80 antigen, Cd28l, B7.1

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Galerie de données de validation

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  FC experiment of BALB/C mouse peritoneal macrophages using 65076-1-Ig

  1X10^6 BALB/c mouse peritoneal macrophages were surface stained with 0.5 ug Anti-Mouse CD80 (B7-1) (65076-1-Ig, Clone:16-10A1) and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100 or unstained. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: macrophages péritonéaux de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

65076-1-Ig cible CD80 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Armenian Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de cellules CHO transfectées avec B7 (CD80) de souris
• Nom complet: CD80 antigen
• Numéro d’acquisition GenBank: BC131959
• Symbole du gène: CD80
• Identification du gène (NCBI): 12519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse	FC	Biomolecules; Glucose Transporter Glut1-Dependent Metabolic Reprogramming Regulates Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages; Authors - Alex Cornwell
mouse	FC	MedComm (2020); Full-active pharmaceutical ingredient nanosensitizer for augmented photoimmunotherapy by synergistic mitochondria targeting and immunogenic death inducing; Authors - Xianghui Li

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