Anticorps Recombinant de lapin anti-CD80

CD80 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241371E10

N° de cat : 84137-5-RR

Synonymes

Ly 53, CD80 antigen, Cd28l, B7.1, B7 1

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Galerie de données de validation

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  WB analysis of mouse spleen using 84137-5-RR

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84137-5-RR (CD80/B7-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84137-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84137-5-RR against Mouse CD80/B7-1 were performed. The affinity constant is 0.204 nM.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84137-5-RR cible CD80 dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD80 antigen
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_009855.2
• Symbole du gène: CD80
• Identification du gène (NCBI): 12519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

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