Anticorps Polyclonal de lapin anti-CD81

• Phare
• Validé par KD/KO

CD81 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(94)

N° de cat : 27855-1-AP

Synonymes

Tetraspanin 28, TAPA1, S5.7, CD81 molecule, CD81 antigen

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Galerie de données de validation

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  WB analysis of Raji using 27855-1-AP

  Jurkat cells and Raji cells were subjected to SDS PAGE followed by western blot with 27855-1-AP (CD81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 27855-1-AP

  WB result of CD81 antibody (27855-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and CD81 knockout HCT 116 cells.

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  WB analysis using 27855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27855-1-AP (CD81 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27855-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27855-1-AP (CD81 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27855-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27855-1-AP (CD81 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 87 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

27855-1-AP cible CD81 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD81 Protéine recombinante Ag27298
• Nom complet: CD81 molecule
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC002978
• Symbole du gène: CD81
• Identification du gène (NCBI): 975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury; Authors - Xun Wu
human	WB	EMBO J; Extracellular vesicles from Zika virus-infected cells display viral E protein that binds ZIKV-neutralizing antibodies to prevent infection enhancement; Authors - Fanfan Zhao
human	WB	J Control Release; 3D mesenchymal stem cell exosome-functionalized hydrogels for corneal wound healing; Authors - Yuehe Xu
rat	WB	J Nanobiotechnology; Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy; Authors - Junlai Wan
rat	WB	Adv Healthc Mater; Dual-Barb Microneedle with JAK/STAT Inhibitor-Loaded Nanovesicles Encapsulation for Tendinopathy; Authors - Minhao Chen
human	WB	Cell Commun Signal; Extracellular vesicular delivery of ceramides from pulmonary macrophages to endothelial cells facilitates chronic obstructive pulmonary disease; Authors - Qiqing Huang

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