Anticorps Polyclonal de lapin anti-CD81

• Phare
• Validé par KD/KO

CD81 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

40

N° de cat : 27855-1-AP

Synonymes

CD81, CD81 antigen, CD81 molecule, Cell and organelle markers, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

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Galerie de données de validation

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  WB analysis of Raji using 27855-1-AP

  Jurkat cells and Raji cells were subjected to SDS PAGE followed by western blot with 27855-1-AP (CD81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27855-1-AP (CD81 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 39 publications below
• IHC: See 2 publications below

Informations sur le produit

27855-1-AP cible CD81 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD81 Protéine recombinante Ag27298
• Nom complet: CD81 molecule
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC002978
• Symbole du gène: CD81
• Identification du gène (NCBI): 975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury; Authors - Xun Wu
rat	WB	J Nanobiotechnology; Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy; Authors - Junlai Wan
mouse	WB	J Control Release; Engineered exosome as targeted lncRNA MEG3 delivery vehicles for osteosarcoma therapy.; Authors - Xin Huang
human	WB	Biomed Pharmacother; A 3D culture system improves the yield of MSCs-derived extracellular vesicles and enhances their therapeutic efficacy for heart repair; Authors - Ling Sun
human	WB	J Pathol; Inflammatory capacity of exosomes released in the early stages of acute pancreatitis predicts the severity of the disease.; Authors - Montserrat Carrascal
mouse	WB	Aging (Albany NY); Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis.; Authors - Mei-Ling Xu