Anticorps Recombinant de lapin anti-CD81

• Phare
• Validé par KD/KO

CD81 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241722B8

N° de cat : 84644-1-RR

Synonymes

TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1

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Galerie de données de validation

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  WB analysis using 84644-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84644-1-RR (CD81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 84644-1-RR

  WB result of CD81 antibody (84644-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and CD81 knockout HCT 116 cells.

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  Affinity and Kinetic Characterization of 84644-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84644-1-RR against Human CD81 were performed. The affinity constant is 0.602 nM.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HCT 116, cellules Jurkat, cellules K-562, cellules Ramos, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84644-1-RR cible CD81 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD81 molecule
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: NM_004356.4
• Symbole du gène: CD81
• Identification du gène (NCBI): 975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

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