Anticorps Recombinant de lapin anti-CD81

CD81 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

5A6

N° de cat : CL750-65644

Synonymes

TAPA1, S5.7, CD81 molecule, CD81 antigen, 5A6

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using CL750-65644

  1x10^6 human peripheral blood leukocytes were surface stained with 5ul CoraLite® Plus 750 Anti-Human CD81 (5A6) Mouse IgG2a RecAb (CL750-65644, Clone: 5A6) or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL750-65644

  1x10^6 human peripheral blood leukocytes were surface stained with 5ul CoraLite® Plus 750 Anti-Human CD81 (5A6) Mouse IgG2a RecAb (CL750-65644, Clone: 5A6) (red) or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue).Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-65644 cible CD81 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lignée cellulaire OCI-LY8 humaine
• Nom complet: CD81 molecule
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: BC002978
• Symbole du gène: CD81
• Identification du gène (NCBI): 975
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

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