Anticorps Polyclonal de lapin anti-CD82

CD82 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10248-1-AP

Synonymes

4F9, C33, C33 antigen, CD82 antigen, CD82 molecule, CD82, KAI1, GR15, IA4, Inducible membrane protein R2, KAI1, Metastasis suppressor Kangai 1, R2, SAR2, ST6, Tetraspanin 27, Tspan 27, TSPAN27

---

Galerie de données de validation

• 

  WB analysis of K-562 using 10248-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10248-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10248-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules K-562, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de la thyroïde humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10248-1-AP cible CD82 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD82 Protéine recombinante Ag0314
• Nom complet: CD82 molecule
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC000726
• Symbole du gène: CD82
• Identification du gène (NCBI): 3732
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

Publications

Species	Application	Title
human	IF	Adv Biol (Weinh); Tetraspanin CD82 Associates with Trafficking Vesicle in Muscle Cells and Binds to Dysferlin and Myoferlin; Authors - Tatiana Fontelonga
human	WB	Oncol Lett; MicroRNA-633 enhances melanoma cell proliferation and migration by suppressing KAI1.; Authors - Zhengxiang Wang
human	IHC	Asian Pac J Cancer Prev; Down Regulation of KAI1/CD82 in Lymph Node Positive and Advanced T-Stage Group in Breast Cancer Patients.; Authors - Thammineni Krishna Latha
mouse	WB	Parasit Vectors; Quantitative proteomic analysis of local and systemic extracellular vesicles during Eimeria falciformis infectious cycle in the host; Authors - Joshua Seun Olajide