Anticorps Polyclonal de lapin anti-CD82

CD82 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10248-1-PBS

Synonymes

CD82, KAI1, CD 82, CD82,KAI1, IA4, Metastasis suppressor Kangai-1

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Galerie de données de validation

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  WB analysis of K-562 using 10248-1-AP (same clone as 10248-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10248-1-AP (same clone as 10248-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10248-1-PBS cible CD82 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD82 Protéine recombinante Ag0314
• Nom complet: CD82 molecule
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC000726
• Symbole du gène: CD82
• Identification du gène (NCBI): 3732
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

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